Kinases with known consensus sequences full#

Should VWR need to supply products and/or services of different specifications, sources, and pack size to that published or ordered by the customer, VWR shall do so only after consultation with and agreement by the customer. The terms and conditions of the contract apply equally to the supply of both products and services except where application to one or the other is specified. The customer’s order for the products and/or services is an offer by the customer to purchase those products and/or services in accordance with these terms and conditions and shall only be deemed accepted when VWR issues written acceptance of the order, at which point the contract between the customer and VWR for the supply of those products and/or services, comes into existence (contract).Īny additional or special terms included by VWR in its written acceptance shall form part of the contract.

Kinases with known consensus sequences full#

VWR reserves the right immediately to cancel any orders from customers who have not complied in full with these procedures. VWR’s products can be hazardous and all customers are required to comply with the VWR account application procedures which are available on request. These terms and conditions apply to the exclusion of any other terms submitted by the customer or which are implied by any trade, custom, practice or course of dealing. The characterization of ICSBP mRNA regulation should help identify functions for this putative IFN trans-acting factor in macrophage activation.These terms and conditions cover all sales of products and services by VWR International Ltd (VWR) in the United Kingdom and any information and advice given whether charged for or not, unless otherwise agreed by VWR in writing. ICSBP mRNA induction is also inhibited by dexamethasone, a synthetic glucocorticoid, well known as an anti-inflammatory drug capable of influencing gene expression in macrophages. IFN-gamma-induction of ICSBP mRNA is resistant to cycloheximide but sensitive to protein kinase inhibitors (H7, H8, HA-1004, staurosporine) at doses that suggest that protein kinase C is a likely target.

IFN-alpha, at a concentration shown previously to modulate macrophage surface markers and functions, had no effect on ICSBP message levels alone, but antagonized the IFN-gamma-induction of ICSBP mRNA.

ICSBP mRNA levels increased within 1 h of IFN-gamma treatment, peaked between 4 and 6 h, and subsequently declined to approach baseline levels by approximately 24 h. Steady-state levels of both of these species were elevated by IFN-gamma at doses consistent with many IFN-gamma-induced macrophage functional responses. Northern blot analysis revealed two ICSBP mRNA species from these cells. In this study, the regulation of ICSBP mRNA induction by IFN-gamma was characterized in murine thioglycolate-elicited peritoneal macrophages. Homologous interferon responsive sequences have been found in many IFN-inducible genes, and the gene for a protein that binds these sequences (interferon consensus sequence binding protein, ICSBP) has recently been cloned. IFN play a central role in the activation of macrophages by inducing the expression of several proteins which, in turn, result in increased functional capabilities.